Lassnigg A, Schmid ER, Hiesmayr M, Falk C, Druml W, Bauer P, et al
Indeed, the insertion of staples forces the peptide into -helical conformation, which enhances the helicity of the peptide and also increases its hydrophobicity by exposing non-polar side chains to the environment outside
Cell culture and bioluminescence analysis T cells, isolated from mice, were activated by CD3/CD28 Streptamer kit (#6-8920, iba, Goettingen, Germany) for 30 min and cultured in RPMI 1640 medium (#8123340, Gibco) containing 10% FBS (#A5669701,Gibco), 1% penicillin/streptomycin, 50 M -mercaptoethanol (Beyotime, Shanghai, China), 1 mM sodium pyruvate (Beyotime) and 100 U/mL interleukin-2 (#CK24, Novoprotein, Suzhou, Jiangsu, China)
The synthesis process carefully manages the incorporation of alpha-aminoisobutyric acid (Aib) at the N-terminus and the D-amino acids (D-2-naphthylalanine and D-phenylalanine), which do not occur in natural proteins but are critical to the peptides final properties
P values for unpaired comparisons between two groups with comparable variance were calculated by two-tailed Students t -test
